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Фундаментальные исследования
ISSN 1812-7339
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ИФ РИНЦ = 1,674

THE STUDY OF MECHANISM OF ANTITUMOUR ACTIVITY OF NEW AMINOACID COMPLEX LITHIUM

Zalalutdinova L.N., Petrov S.V.,Jadukov O.A, Husainova L.F., Zueva N.A., Imanaeva E.R., Valeeva A.V.

Many complex compounds of essential elements copper, vanadium, iron, lithium and other show antitumour activity in vivo and in vitro, and can become the perspective class of antiblastic drugs. On department of pharmacology KSMU are studied the antitumour properties of new original complexes of trace elements with amino acid and oligopeptides, which synthesized by A.V.Zacharov, В.G.Zhturlin and others in Kazan State University. The amino acid complexes lithium were most active and small toxicity in experiments. So we exude the expedient study of mechanism of antitumour activity of new amino acid complex lithium.

In this work were shown the results of study of influence compound on growing of tumour cells in vitro. As experimental model is used celluar line rhabdomyosarcoma human (RD). Monocoat celluar culture support on culture medium Igla MEM with addition fetal calf serum. The cells separated with tripsin and versen in correlation 1:1. Amino acid complex lithium was entered in celluar culture 7,5 microg on ml culture medium that corresponds to 0,5 ´ 10-4 mol/l. The estimation of results of influence of new compound on cancer cells of culture we conducted immunohistochemical with avedin-biotin methods for period 48 h on cell cycle progression.

In work were used the monoclonal antibody (DAKO) to oncomarkers, markers of differentiation and proliferation of cells: bcl-2, p53, myogenin, desmin, Ki-67.

There was is stated that single cells of intact rhabdomyosarcoma expression myogenin that points an differentiation of cells of tumor to comparatively high degree. New amino acid complex lithium greatly does not influence upon expression of myogenin.

In realization of mechanism of antitumour activity of new amino acid complex lithium plays importance possible breach cytoskelet of cells, revealled on change the stain on desmin; as well as braking an proliferation, about than witnesses change the nature to reactions on Ki-67 - nucleus marker an proliferation. Like celluar change pack in mechanism of ruins of cell on apoptosis way. The disappearance bcl-2- protein that inhibitor of apoptosis arguments in favour of this in cells, cultivated with new compound, and presence р53 in these cells. Generally accepted that disbalans between these proteins aside increase р53 leads away the cell in apoptosis.

Thereby, one of the the probable mechanisms of action of new amino acid complex lithium, showing antiblastomic activity in experiments in vivo, is induction apoptosis.


Библиографическая ссылка

Zalalutdinova L.N., Petrov S.V.,Jadukov O.A, Husainova L.F., Zueva N.A., Imanaeva E.R., Valeeva A.V. THE STUDY OF MECHANISM OF ANTITUMOUR ACTIVITY OF NEW AMINOACID COMPLEX LITHIUM // Фундаментальные исследования. – 2005. – № 8. – С. 76-77;
URL: https://fundamental-research.ru/ru/article/view?id=6468 (дата обращения: 01.12.2024).

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«Фундаментальные исследования» список ВАК ИФ РИНЦ = 1,674