Цель: выявить частоту инфицирования H.pylori у больных хронической HCV-инфекцией. Было обследовано 25 больных (мужчин – 16 и женщин – 9) с хронической HCV-инфекцией. Всем больным была выполнена фиброэзофагогастродуоденоскопия с биопсией слизистой оболочки желудка (антральный отдел) и двенадцатиперстной кишки. Диагностика H.pylori проводилась уреазным тестом («Хелпил», АMA, С.Петербург), ПЦР по методике «Литех» (Москва) и по составу выдыхаемого воздуха с помощью теста «Хелик» (АМА, С.-Петербург) индикаторными трубками. Инфицированность H.pylori была выявлена с помощью уреазного теста и методом ПЦР во всех биоптатах слизистой оболочки желудка и 12-перстной кишки. Чувствительность дыхательного теста cоставила 67,57 %. Выявлены изменения пищевода, желудка и 12-перстной кишки, ассоциированные с H.pylori инфекцией, у больных хроническим вирусным гепатитом С. H.pylori инфекция может являться фактором риска формирования более тяжелых исходов в течении HCV-инфекции, что требует широкого внедрения скрининговых методов диагностики среди больных хронической HCV-инфекцией.
Aim: To determine the prevalence of H.pylori – infection among patients with chronic HVC – infection. 25 patients (16 men, 9 women) were examined. The esophagogastrodudodenoscopy with biopsy of gastric mucosa (antrum) and duodenum was carried out among patients. Diagnosis of H.pylori was carried out by means of the urease test («Helpyl», AMA, S.-Petersburg), by PCR(«Helicopol», «Lytech», Moscow) and by urea breath test («Helic», AMA, S.-Petersburg) which had determinated the expired air’s composition by using Helic-tubes. The positive detection of H.pylori was determined by means of the urease test and by PCR in all gastric and duodenal biopsy speciments. The sensitivity of urea breath test was 67,57 %. Disorders of esophagus, stomach and duodenum which associated with H.pylori was carried out among patients with chronic HCV-infection. H.pylori may be the positive risk factor of more severe complications’ development what requires the large introduction of diagnostic screening methods among patients with chronic HCV-infection.
1. Alter MJ. Epidemiology of hepatitis C // Hepatology. – 1997. – Vol. 26. – P. 62S.
2. El-Masry S. Helicobacter pylori and hepatitis C virus coinfection in Egyptian patients. / El-Masry S., El-Shamat M., Badra G. Aboel-Nour M.F., Lotfy M. // J Glob Infect Dis. – 2010. – Vol.1 (2). – P. 4–9.
3. Esmat G. Role of Helicobacter pylori in patients with HCV-related chronic hepatitis and cirrhosis with or without hepatocellular carcinoma: possible association with disease progression / Esmat G., El-Bendary M., Zakarya S., Ela M.A., Zalata K //J Viral Hepat. – 2012. – Vol. 19(7). – P. 473–479.
4. Furusyo N. Treatment for eradication of Helicobacter pylori infection among chronic hepatitis C patients / Furusyo N., Walaa A.H., Eiraki K., Toyoda K., Ogawa E., Ikezaki H., Ihara T., Hayashi T., Kainuma M., Murata M., Hayashi J. // Gut Liver. – 2011. – Vol. 5(4). – P. 447–453.
5. Ito K. Adherence, internalization, and persistence of Helicobacter pylori in hepatocytes. / Ito K., Yamaoka Y., Ota H., El-Zimaity H., Graham D.Y. // Dig. Dis. Sci. – 2008. – Vol. 53(9). – P. 2541–2549.
6. Konturek S.J. Progastrin and its products from patients with chronic viral hepatitis and liver cirrhosis / Konturek S.J., Gonciarz M., Gonciarz Z., Bielanski W., Mazur W., Mularczyk A., Konturek P.C., Goetze J.P., Rehfeld J.F. // Scand. J Gastroenterol. – 2003. – Vol. 38. – P. 643–647.
7. Mamun-Al-Mahtab. State of Globe: Helicobacter pylori and hepatitis C together hamper health // Glob Infect Dis. – 2010. – Vol. 2 (1). – P. 1–3.
8. Olivera-Severo D. Ureases display biological effects independent of enzymatic activity. Is there a connection to diseases caused by urease-producing bacteria? / Olivera-Severo D., Wassermann G.E., Carlini C.R. // Brazilian Journal of Medical and Biological Research. – 2006. – Vol. 39. – P. 851–861.
9. Pellicano R. Helicobacter pylori seroprevalence in hepatitis C virus positive patients with cirrhosis. / Pellicano R., Leone N., Berrutti M., Cutufia M.A., Fiorentino M., Rizzetto M., Ponzetto A. // Journal Hepatology. – 2000. – Vol.33. – P. 648–650.
10. Ponzetto A. Helicobacter pylori infection in patients with hepatitis C virus positive chronic liver diseases. / Ponzetto A., Pellicano R., Pedaelli A., Rizzetto M., Roffi L// Microbiologie. – 2003. – Vol. 26. – P. 321–328.
11. Rocha M. Association of Helicobacter species with hepatitis C cirrhosis with or without hepatocellular carcinoma. / Rocha M., Avenaud P., Menard A., Le Bail B., Balabaud C., Bioulac-Sage P., de Magalhães Queiroz D.M., Mégraud F // Gut. – 2005. – Vol. 54. – P. 396–401.
12. Silva L.D. The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient. /Silva L.D., Rocha A.M., Rocha G.A., de Moura S.B., Rocha M.M., Dani R., de Melo F.F., Guerra J.B., de Castro L.P., Mendes G.S., Ferrari T.C., Lima A.S., Queiroz D.M.//Mem Inst Oswaldo Cruz. – 2011. – Vol. 106(6). – P. 748–54.
13. Stalke P. Detection of Helicobacter species in liver and stomach tissues of patients with chronic liver disease using polymerase chaine reaction- denauring gradient gel electrophoresis and immunohistochemistry. / Stalke P., Abu Al-Soud W., Bielawski K.P., Bakowska A., Trocha H., Stepinski J., Wadström T. // Scand. J. Gastroenterol. – 2005. – Vol. 40. – P. 1032–1041.
14. Suganuma M. Helicobacter pylori membrane protein 1: a new carcinogenic factor of Helicobacter pylori. / Suganuma M., Kurusu M., Okabe S., Sueoka N., Yoshida M., Wakatsuki Y., Fujiki H. // Cancer Res. – 2001. – Vol. 61. – P. 6356–6359.
15. Tian X.F. A two-year animal experimental study on the pathological effects of Helicobacter pylori on liver tissues. / Tian X.F., Fan X.G., Huang X., Fu C.Y., Dai H., Huang Y. // Zhonghua Gan Zang Bing Za Zhi. – 2008. – Vol. 16(2). – P. 129–33.